陳家良 博士 高聘研究員 博（碩）士生導師

  郵箱：jialiangchen@hainanu.edu.cn

 辦公地址：海南大學美安藥學大樓345-5

 1.教育及工作背景

 2024-至今 海南大學 365英国上市官网在线 高聘研究員

 2022-2024 棗莊學院 化學化工與材料科學學院 副教授

 2019-2022 南開大學 化學學院 博士後

 2017-2019 南開大學 生命科學院 博士後

 2010-2016 南開大學 化學院 有機化學博士

 2006-2010 山東師範大學 化學化工與材料科學學院 理學學士

2.科研情況

圍繞蛋白質溶液動态結構與功能關系研究中的技術難點，發展順磁核磁共振新方法，并用于闡釋多結構域蛋白質在溶液中的動态結構變化及其與功能的關系、蛋白酶在催化反應過程中短壽命中間體的三維結構及催化反應分子機制；利用順磁核磁共振技術分析藥物分子和天然産物的立體化學結構。近年來發表SCI論文20餘篇，其中以第一作者在Angew. Chem. Int. Ed., ACS Catal., Sci. China Chem., J. Phy. Chem. Lett., Chem. Commun.等期刊發表多篇論文。主持國家自然科學基金面上、青年基金項目、山東省青創團隊項目，2017年入選全國博士後“博新計劃”項目。

3.研究領域

蛋白質動态結構-功能關系、基于蛋白質動态結構的藥物分子篩選、代謝物定量分析檢測、手性藥物分子立體構型分析

4. 社會兼職

Analytical Chemistry, Biosensors & Bioelectronics等學術期刊審稿人

5.代表性項目

1）國家自然科學基金面上項目（22374126）：利用赝接觸位移确定手性有機分子的立體構型，2024-2027，46萬，主持；

2）國家自然科學基金青年項目（22004072）：細胞内定點标記蛋白質并應用于高分辨生物磁共振研究，2021-2023，24萬，主持；

3）山東省高校“青創團隊計劃”項目（2022KJ279）：重大疾病代謝物的多模态原位定量分析，2023-2025，30萬，主持；

4）2017年全國博士後創新人才支持計劃-“博新計劃”（BX201700124），60萬，主持。

6.代表性成果

1) Jia-Liang Chen, Bin Li, Feng Yang, Tiesheng Shi, Xun-Cheng Su. Increase of chiral sensing ability in host-guest chemistry by magnetic anisotropy. Sci. China Chem., 2024, 67, 2775-2782.

2) Jia-Liang Chen, Xiao Wang, Feng Yang, Bin Li, Gottfried Otting, Xun-Cheng Su. 3D structure of the transient intermediate of the enzyme−substrate complex of sortase A reveals how calcium binding and substrate recognition cooperate in substrate activation. ACS Catal., 2023, 13, 11610-11624.

3) Jia-Liang Chen; Yin Yang, Tiesheng Shi, Xun-Cheng Su. Effective assessment of lanthanide ion delivery into live cells by paramagnetic NMR spectroscopy. Chem. Commun., 2023, 59, 10552-10555.

4) Ya-Ting Chen, Bin Li, Jia-Liang Chen, Xun-Cheng Su. Stereospecific recognition of a chiral centre over multiple flexible covalent bonds by ¹⁹F-NMR. Analyst, 2023, 148, 233-238.

5) Jia-Liang Chen, Bin Li, Bo Ma, Xun-Cheng Su. Distinct stereospecific effect of chiral tether between a tag and protein on the rigidity of paramagnetic tag. J. Biomol. NMR, 2022, 76, 107-119.

6) Bo Ma†, Jia-Liang Chen†, Chao-Yu Cui†, Feng Yang, Yan-Jun Gong, and Xun-Cheng Su. Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis. Chem. Eur. J. 2021, 27, 16145-16152.

7) Jia-Liang Chen, Ben-Guang Chen, Bin Li, Feng Yang and Xun-Cheng Su. Assessing multiple conformations of lanthanide inding tags for proteins using a sensitive ¹⁹F-reporter. Chem. Commun. 2021, 57, 4291-4294.

8) Ya-Ting Chen†, Bin Li†, Xia-Yan Li, Jia-Liang Chen, Chao-Yu Cui, Kaifeng Hu, Xun-Cheng Su. Simultaneous identification and quantification of amino acids in biofluids by reactive ¹⁹F-tags. Chem. Commun. 2021, 57, 13154-13157.

9) Wence Xu†, Guilan Fan†, Jialiang Chen, Jinhan Li, Le Zhang, Shengli Zhu, Xuncheng Su, Fangyi Cheng and Jun Chen. Nanoporous palladium hydride for electrocatalytic N₂ reduction under ambient conditions. Angew. Chem. Int. Ed. 2020, 59, 3511-3516.

10) Qiang Li, Jialiang Chen, Marko Trajkovski, Yifei Zhou, Chaochao Fan, Kuan Lu, Pingping Tang, Xuncheng Su, Janez Plavec, Zhen Xi and Chuanzheng Zhou. 4′-Fluorinated RNA: synthesis, structure, and applications as an ultra-sensitive ¹⁹F NMR probe of RNA structure and function. J. Am. Chem. Soc. 2020, 142, 4739-4748.

11) Jia-Liang Chen†, Bin Li†, Xia-Yan Li, and Xun-Cheng Su. Dynamic exchange of the metal chelating moiety: a key factor in determining the rigidity of protein−tag conjugates in paramagnetic NMR. J. Phys. Chem. Lett. 2020, 11, 9493-9500.

12) Xun-Cheng Su*, Jia-Liang Chen. Site-specific tagging of proteins with paramagnetic ions for determination of protein structures in solution and in cells. Acc. Chem. Res. 2019, 52, 1675-1686.

13) Jia-Liang Chen, Xiao Wang, Yu-Hao Xiao and Xun-Cheng Su. Resonance assignments of lowly populated and unstable enzyme intermediate complex under real-time conditions. ChemBioChem, 2019, 20, 2738-2742.

14) Mingming Li†, Yashan Ning†, Jialiang Chen, Xingchen Duan, Na Song, Dan Ding, Xuncheng Su, and Zhilin Yu. Proline isomerization-regulated tumor microenvironment adaptable self-assembly of peptides for enhanced therapeutic efficacy. Nano Lett. 2019, 19, 7965-7976.

15) Xiao Wang, Jia-Liang Chen, Gottfried Otting, Xun-Cheng Su. Conversion of an amide to a high-energy thioester by Staphylococcus aureus sortase A is powered by variable binding affinity for calcium. Sci. Rep. 2018, 8, 16371.

16) Jia‑Liang Chen†, Yu Zhao†, Yan‑Jun Gong, Bin‑Bin Pan, Xiao Wang, Xun‑Cheng Su. Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis. J. Biomol. NMR, 2018, 70, 77-92.

17) Yin Yang†, Feng Yang†, Yan-Jun Gong, Jia-Liang Chen, Daniella Goldfarb, and XunCheng Su. A reactive, rigid Gd^III labeling tag for in-cell EPR distance measurements in proteins. Angew. Chem. Int. Ed. 2017, 56, 2914-2918.

18) Jia-Liang Chen, Xiao Wang, Feng Yang, Chan Cao, Gottfried Otting, and Xun-Cheng Su. 3D structure determination of an unstable transient enzyme intermediate by paramagnetic NMR spectroscopy. Angew. Chem. Int. Ed. 2016, 55, 13744-13748.

19) Jia-Liang Chen, Yin Yang, Lin-Lin Zhang, Haobo Liang, Thomas Huber, Xun-Cheng Su and Gottfried Otting. Analysis of the solution conformations of T4 lysozyme by paramagnetic NMR spectroscopy. Phys. Chem. Chem. Phys. 2016, 18, 5850-5859.

20) Fei-He Ma†, Jia-Liang Chen†, Qing-Feng Li†, Hui-Hui Zuo, Feng Huang, and Xun-Cheng Su. Kinetic assay of the michael addition-like thiol–ene reaction and insight into protein bioconjugation. Chem. Asian J. 2014, 9, 1808-1816.

歡迎分子生物學、核磁共振、化學生物學、合成化學背景的研究生和博士加盟！

歡迎本科生來課題組進行科研訓練！